Device

Part:BBa_K4491010:Design

Designed by: Yuen Shan Ho   Group: iGEM22_Cambridge   (2022-10-11)


Autoregulatory Negative feedback circuit


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 18
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 460
    Illegal BamHI site found at 4
    Illegal BamHI site found at 42
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

NA


Source

We have designed an autonegative feedback loop (nFc) to study the effect of adaptation. An autoregulatory negative feedback refers to a motif that involves a species feeding back to downregulate itself. The feedback species we have chosen is VanR and the promoter we have chosen is P_VanCC, the effect of VanR on P_VanCC at different concentration of Vanillic acid pf inducer is well characterised by Meyer et al., (2019). Essentially, VanR will act on P_VanCC, repressing its production, following a Hill Function dynamics. In order to characterise the amount of vanR, we have created an operon (MegaT) with a RBS, mVenus and Terminator after VanR coding sequence (CDS).

References